Conference Schedule

Day1: May 14, 2018

Keynote Forum

Biography

Dr. Toomer is employed with the USDA-ARS as a Research Chemist in the Market Quality and Handling Research Unit and as an Adjunct Professor in the Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh NC. She graduated from North Carolina State University with a Ph.D. in Nutrition with a minor in Biotechnology in 2005. In July 2005, Toomer began a postdoctoral fellowship in the Mucosal Immunology Laboratory of Pediatric Gastroenterology at Massachusetts General Hospital/Harvard Medical School under the supervision of Dr. Allan Walker and Dr. Hai Ning Shi. Toomer completed a second postdoctoral training program at the United States Department Agriculture (USDA)/Agricultural Research Service (ARS) in Beltsville MD with a research emphasis in differential gene expression in the reproductive tissues of turkey hens. Subsequently, Dr. Toomer was employed for over 7 years with the United States Food and Drug Administration-Center for Food Safety and Applied Nutrition as a Research Biologist with a research emphasis in the development and prevention of Pediatric Food Allergy and the identifying immunological biomarkers of food allergy using in vitro and in vivo models, and studying the effects of commensal microorganisms and/or probiotics on early (pre-weaning) immunological development.


Abstract

Peanuts, Arachis hypogaea, are one of the most widely consumed legume globally due to its nutrition, taste and affordability. Peanuts are protein and energy-rich and have been utilized worldwide to address the nutritional needs of people and production animals in developing countries. In the United States, 85% of the American peanut production is utilized for domestic edible products, such as peanut butter, candy, confections, and snacks. Thus, our goal in MQH-ARS-USDA has been to evaluate the chemical constituents of peanuts and to investigate the usefulness of peanuts and/or peanut products as a feed and/ or food ingredient. We aimed to identify the usefulness of new high-oleic peanut cultivars as a preferable feed ingredient for poultry. Previous studies have identified normal-oleic peanuts as a suitable and economical poultry feed ingredient. However, no studies to date have examined the use of high-oleic (HO) peanut cultivars as a feed ingredient for poultry and determined the impact of feeding. HO peanuts on poultry performance or nutritive qualities of the eggs produced. To meet these objectives layer hens were fed a conventional diet (soybean meal + corn) or a HO peanut + corn diet for 10 weeks. Body and feed weights were collected weekly. Pooled egg samples were analyzed for quality and lipid analysis. There were no significant differences between treatment groups in hen performance or egg quality. Eggs produced from layer hens fed the HO peanut diet had significantly (p<0.05) greater yolk color scores (2-fold), HO fatty acid and β-carotene levels in comparison to the controls. Eggs produced from hens fed the control diet had significantly greater content of saturated fatty acids and trans fat in comparison to eggs produced from layer hens fed the HO peanut diet. This study identifies HO peanuts as an abundant commodity that could be used to support local agricultural markets of peanuts and poultry and be of economic advantage to producers while providing a potential health benefit to the consumer with improved egg nutrition. Secondly, we aimed to determine the usefulness of peanut skins, a significant waste product produced within the peanut industry, as a functional food ingredient. Numerous studies have demonstrated the health promoting benefits of phenolic compounds found in antioxidant rich plant foods such as, green tea, blueberries, broccoli, beets, seaweed, spinach and grape seeds shown to lower serum and liver low-density lipoproteins, prevent free radical DNA damage and to reduce histamine release and inflammation. To meet our objectives mice were fed a control mouse chow diet (A), an atherogenic diet (B) or an atherogenic diet supplemented with peanut skin phenolic extract (C) for 20 weeks. Bodyweights and blood samples were collected weekly and at termination, liver samples were collected for analysis. Mice fed the peanut skin phenolic extract supplemented atherogenic diet (C) had reduced hepatic inflammation and blood glucose levels in comparison to treatment B, suggesting that phenolic extracts from peanut skins like ther naturally occurring plant compounds may have health benefits when consumed in the diet.

Biography

Massimo Zonca is a Food Technologist graduated from Milan State University. He has 30+ years of experience in leading Packaging Development, Food Process and Innovation at H J Heinz, and also at Kraft Heinz Company. Since 2016, he is an Independent Consultant on Food Packaging and Food Process, based in Bergamo, Italy. He held various roles at Heinz, as Packaging Engineer & Development Manager (1987- 2006), European R&D Manager (2006-2009) and Global Packaging Innovation Efficiency Manager until February 2016, managing global initiatives on packaging and innovation and facilitate technology transfer. He gained expertise working in several international projects both in Food Packaging Development and Food Process. He managed food contact issues working together with the DG SANCO (European Commission) and the Istituto Superiore di Sanita- ISS (Italian Health Ministry). He is a member of GSICA, Italian Scientific Group for Food Packaging, does lectures in Parma University on Food Packaging and provides speech at major conferences. He served his hometown (Filago – BG) as a Mayor, from 2005 to 2015. 


Abstract

Ohmic heating offers a valid alternative to conventional heating, especially for product with big particulates (up to 30 mm), because it simultaneously heats both phases by internal energy generated through electrical energy. In this process, heating rate depends upon the electrical conductivity and field strength. This technique grants a minimum structural damage to a product, which retains its nutritional value and gives excellent processed quality products in minimum operating time, as for HTST process - High Temperature Short Time. This technology was facing several issues during application in terms of process control (appropriate temperature increase control, electrical arc generation, possible burning of product, etc.): those issues discouraged food technologist and technician to promote this technology and, to date, some preconceptions still be present and often become an obstacle for a deep evaluation and adoption of Ohmic heating. The aim of this work is to demonstrate how the problems on this technology were fixed, thanks to the determination from an Italian company, focusing on electrode geometry, application of high frequency, modularity of the equipment and software control; also, the presentation will make an excursus and provide an overview of the most recent applications on food with particulates or specific food with very sensitive components/ingredients and then packed in aseptic condition. Results will be presented taking real examples from the 40 plus industrial equipment installed worldwide and the most recent applications. For each application the main process data: capacity, power and tension applied, flow rate, particulate dimensions, pumping systems, pipe diameter will be showed together with some equipment details. 

Biography

Sandra Poirier has been employed at Middle Tennessee State University, USA, since 2005. She received her BSc and MSc degrees in Family and Consumer Sciences from Florida State University and University of Arizona respectively. Her Doctorate was received in 1998 from Florida International University in Miami, Florida with her dissertation in the area of health-related quality of life. Prior to coming to MTSU, she taught for three years at Zayed University in Dubai, United Arab Emirates. Additionally, she has worked at the University of Florida Cooperative Extension Service, Florida International University, Florida Atlantic University, University of Arizona Cooperative Extension Service, and the Provincial Government of Alberta, Canada. She has more than 15 years of international teaching experience working in culturally diverse environments. Her strengths include creating innovative educational programs with a focus on food and culture, identifying appropriate outreach efforts to solve community problems, and empowering students for successful careers. She has been recognized for her ability to create and teach online courses, work as an advisor for student organizations, and creating positive educational environments for optimal learning. She has published over 25 journal articles and been invited to speak in Malaysia, Switzerland, Germany, Poland, Austria, Puerto Rico, and the Andros Island in the Caribbean. 


Abstract

Rising obesity, food insecurity, a lack of quality education and safe water for the poor in the developing world are some of the global society’s entrenched problems. Whose job is it any way to solve these problems? For decades, the answer to the question has been simple: government. Today we live in a different world where a new economy has emerged. The new “solutions economy” represents not just an economic opportunity, but a new strategy for solving many of the global nutrition and health problems. This presentation explores how, in today’s new “solutions economy,” solving social problems is becoming a multidisciplinary exercise that challenges businesses, governments, philanthropists, and social enterprises to think holistically about their role and their relation to others, not as competitors but as collaborators serving as many stakeholders as possible.

Biography

Yiannis Kourkoutas has completed his PhD from University of Patras, Greece, and Postdoctoral Studies from University of Ulster, Northern Ireland, UK. He is the Director of Laboratory of Applied Microbiology & Biotechnology at the Department of Molecular Biology & Genetics, Democritus University of Thrace, Greece. He has published more than 80 papers in peer-reviewed journals and has been serving as a lead Guest Editor and an Editorial Board Member in several special issues and reputed scientific journals.


Abstract

Nowadays there is a growing interest in developing novel functional foods containing probiotic microorganisms. To deliver the health benefits, probiotic products need to contain an adequate amount of live bacteria capable of surviving the acidic conditions of the upper gastro-intestinal tract, as well as proliferating in the intestine, a requirement that is not always fulfilled. To overcome such deficiencies, immobilization techniques have been developed, aiming at stabilization of cells and formulation of new types of foods fortified with immobilized health-promoting bacteria that are only released upon reaching the human gut. In this vein, immobilization of probiotic cells on natural, food-grade, prebiotic dietary fibers was studied. As it is important to develop approaches that are compatible with the use of dried cultures, a technology which accommodates its use by the industrial and commercial sector, the wet immobilized cultures were then subjected to drying processes (freeze- and thermal-drying) and the survival rate after the drying procedure and during storage at room and low-temperatures (4 and -18oC) for various time periods (up to 1 year) were studied. Cell immobilization
enhanced cell viability and extended the shelf-life of pro-and pre-biotic fortified foods, as documented by microbiological and molecular analysis. The probiotic strains were encountered at levels greater than 7 logcfu/g after 2 and 12 months of storage at ambient and low temperatures, respectively, and can also withstand high temperature processing steps (up to 60oC) with no significant loses. Our process resulted in successful dried immobilized probiotic culture preparations appropriate for many food applications that maintained cell viability during storage for time periods suitable for industrial uses.

Tracks

  • Food safety | Food additives | Nutraceuticals | Food Chemistry | Food waste management | Food quality standards | Food packaging | Food and human health | Horticulture
Location: Olympica 1

Ondulla T Toomer

USDA-ARS, USA

Chair

Massimo Zonca

Expert in Food Packaging and Food Process, Italy

Co Chair

Biography

Rodríguez-Buenfil Ingrid has her expertise in Biotechnological Process and Design of Experiments. She has 32 years of experience in scientific research, with participation in 78 projects (31 as Project Manager), 4 patents granted and 6 patent applications in process. She has 122 publications among scientific articles, extensive memoirs, books, book chapters, among others. She has guided 34 graduate students. She was a Founding Director of the Southeast Unit of Center for Research and Assistance in Technology and Design of the State of Jalisco (CIATEJ) in Mérida Yucatán, from July 2002 to April 2015. She has been elected to be a Member of the National Research System (MX) level I from 2017-2020 and a Certified Evaluator of the National Council of Science and Technology (CONACYT). Her current project is, ‘Analysis of the metabolomic changes during the development of the fruit Capsicum chinense jacq cultivated in different soil types (Project CONACYT 257588).


Abstract

Habanero pepper (Capsicum chinense) is the main horticultural species commercially exploited in the Yucatán Peninsula in Mexico, in addition to being a symbol of pungency. It possesses characteristics of commercial interest due to its high contents of capsaicinoids accumulated in the fruit. The contents of these compounds are believed to vary in conditions of hydric or water stress. The fruits of Capsicum chinense plants grown in the Yucatan Peninsula have its appellation of origin based on their unique characteristics, which are suggested to be due to the particularities of the soils in which they are cultivated. For its cultivation three main types of soil are used: K áankablu um (red soils), Box lu um (black soils) or ch ich  lu um (brown soils). The interaction of the soil with the plant affects the development of the fruit, since the amount of nutrients, water and salinity in the soil has a significant effect on the quantity and size of fruit, and in the content of some secondary metabolites with the same factors. The objective of the present work was to determine the content of capsaicinoids and vitamin C in habanero pepper grown in different types of soils and to evaluate the relationship of these contents with the state of maturity of the pepper fruit. There were significant differences in both, the content of capsaicinoids and vitamin C due to different types of soil and the degree of ripeness of the fruit, the highest content of capsaicinoids were obtained with the orange ripened pepper (O) and red soil (figure 1). For the content of vitamin C, the highest amount was found in the red soil in the orange ripened pepper (O) and in those of incomplete ripeness (IR) without significant differences between them. The green immature pepper (V) presented the lowest vitamin C content

Biography

Meor Hussin A S has completed her PhD from International Islamic University, Malaysia and did her Postdoctoral studies (visiting scientist) from Institute of Food Research, UK for 2 years. She is Head of the Department of Food Science for the Faculty of Food Science and Technology, University Putra Malaysia. She has published more than 40 papers in reputed journals.


Abstract

Introduction: The presence of biologically active volatile compounds in Torch ginger (Etlingera elatior) highlighted as a new oriental in flavour and fragrances. Research on bioactive and volatile compounds of torch ginger offers promising development of natural resources into nutraceuticals, cosmeceuticals and biopharmaceuticals. The aim of the study is to characterize the chemical constituents and bioactive compound activities of essential oil from torch ginger extracted using the subcritical carbon dioxide.
Materials & Methods: Essential oil from sliced oven dried tight bud torch ginger flowers were extracted using subcritical carbon dioxide extraction. The bioactive compounds in the essential oil were identified using Gas Chromatography-Mass spectrometry (GCMS). The bioactive compound activities of the essential oils were analysed by total phenolic content (TPC) and total flavonoid content (TFC), antioxidant analysis (DPPH assay, FRAP assay and ABTS scavenging activity) and antibacterial analysis (disc diffusion method and minimal inhibitory concentration (MIC)).
Results: The yield of essential oil obtained from subcritical carbon dioxide extraction was 5.5% and the most abundant compounds in torch ginger included á-pinene, caryophyllene, 1-dodecanol, dodecanoic and dodecanal. The bioactive compound activities of essential oil from torch ginger flower were as such: TPC (1590.23±3.74 mg GAE/100g), TFC (1686.19±17.34 mg QE/100g), DPPH assay (90.04±0.25%), FRAP assay (8876.46±36.35) and ABTS scavenging activity (81.77±2.10%). The inhibition zone exhibited by torch ginger flower’s essential oil shows against several Gram positive bacteria such as Bacillus cereus (40.52±1.28 mm), Listeria monocytogenes (20.51±1.02 mm) and Staphylococcus aureus (9.14±0.51 mm) and against one Gram negative bacteria Klebsiella pneumoniae (30.44±0.90 mm) tested in the study.
Conclusion: These findings clearly indicated that the extracts and essential oil derived from torch ginger flower could be potentially used as a new source of natural antioxidant and antibacterial in the food and pharmaceutical products.

Biography

Babak Rasti has completed his PhD in Food Technology from University Putra Malaysia. He is a Senior Lecturer of Food Technology and Bioprocessing at University Malaysia Sabah (UMS). He has published more than 14 papers in reputed journals


Abstract

The aim of present study was to optimize the extraction condition of K. alvarezii and S. polycystum resulting in the highest antioxidant activity. The extraction conditions, namely extraction time (X1: 3-24 hours) and ethanol concentration (X2: 0-100%), were optimized by employing Response Surface Methodology (RSM). Each of the seaweed has 14 extraction conditions generated by Central-Composite Design to obtain maximum yield (Y1), maximum Total Phenolic Content (Y2), maximum Ferric Reducing Ability of Plasma (Y3), and minimum EC50 of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging ability (Y4). All dependent variables were fitted into second-order polynomial equations of reduced model and showed no significant (p>0.05) lack of fit. The optimal extraction conditions for K. alvarezii and S. polycystum were found to be at 24 hours with 100% ethanol and 16.6 hours with 0% ethanol, respectively. The yield, TPC, FRAP, and EC50 of optimum extraction of K. alvarezii was 0.25±0.01 g, 15.86±0.02 mg GAE/g extract, 72.18±0.04 mM TE/g extract, and 1.12±0.01 mg/ml, respectively, while for S. polycystum extract the values were 0.86±0.01 g, 17.85±0.05 mg GAE/g, 75.70±0.15 mM TE/g extract, and 0.30±0.01 mg/ml, respectively. The final reduced models were fitted by RSM, and hence, the models were accepted and exhibited high antioxidant activity. Overall, the studied seaweeds found to be a good source of antioxidants.

Biography

Daniel Laguado is a Chemical Engineer from Universidad del Valle (Colombia) with a Master degree in Product Formulation and Technology from Universidad de Huelva (Spain). In 2009, he was awarded by the European Union with a scholarship for his Master degree in Engineering Rheology as part of the Erasmus Mundus program. Currently he is working as Research & Development Manager for the consumer health care division in Quala SA, a consumer goods manufacturing industry with presence in several countries in Latin America. He has more than eight years of experience in product & process development working at companies like Johnson & Johnson (Hair, Skin Care and OTC divisions) and PepsiCo (Snacks products). 


Abstract

This work describes the industrial development of a new dietary supplement based on the needs of the Colombian people. Actually, in Colombia, some dietary supplements comes in granules traditionally produced by extrusion; this production process leads to product degradation specially in the vitamin content due to high temperatures and high shear. To solve this issue producers overdose the ingredients to overcome the loss generated due to processing conditions that at the end increases the product cost. Therefore, Quala S.A. proposed a top-spray production technology for continuous fluid bed agglomeration, which allows using lower temperatures with lower shear rates for the granulation leading to less impact on the stability of the vitamins and other components as flavours. The variables of greatest relevance in the production of the multivitamin granules were also studied in order to obtain the main characteristics that are useful for the product consumption. As far as we know, this is an innovative technology for the production of dietary supplements and provides good results not only for Quala S.A. but also for the national industry.

Biography

Maria Paciulli has completed her MSc and PhD in Food Science and Technology at the University of Parma, Italy. She is currently working as a Postdoctoral Fellow in the Department of Food and Drug of the same University, in the area of Food Technology. Her research activity is primarily focused on the investigation of the effect of processing and formulation on the physio-chemical and thermal properties and stability of food products, with a particular focus on oil and fats, bakery products and vegetables. She is an author of 24 papers in reputed journals and 3 book chapters, along with participation in several national or international conferences.


Abstract

In the last decade, research dealing with the use of chestnut has received considerable interest due to chestnut’s promising potential to produce high quality and healthy products with high nutritional value. It can be consumed as fresh, boiled, or roasted. Besides, chestnut is also ground into flour which can be used in the production of diverse high quality products such as bread, cookie, cake, breakfast cereals and also pasta. A new perspective may be the use of the chestnut pericarp, now considered a waste and that represents from 6 to 10 % of the fresh fruit weight, in formulations for cereal-based products. The use of this by-product, naturally rich in antioxidants and fibers, may represent a way to enhance the chestnut supply chain, making it more sustainable, as well as giving an improved nutritional contribution to the chestnut products. In the present research work, feasibility of the addition of chestnuts’ pericarp in several cereal-based products was studied, particularly bread, biscuits and pasta. Studied products were prepared both with wheat flour and in a glutenfree version. Chestnut peels showed a great water absorption capacity with great effects on dough rheological properties regardless the product. Similarly, chestnut peels deeply influenced the appearance of the enriched products with a darker colour and the presence of brown particulates. Moreover, chestnut peels enhanced also total antioxidant content of all tested products. In general, chestnut peels showed many challenges in gluten-free formulations as it competed with other thickening agents for water and hindered the formation of a network responsible of the final product structure. Finally, considering all the tested products, the optimal chestnut peels addition varied from 3 to 7g/100g of the recipe.

Biography

Arezoo Erfanian has completed her PhD in Food Technology from University Putra Malaysia. She is currently a Senior Lecturer at the Faculty of Food Science and Nutrition, University Malaysia Sabah, Malaysia. She has published manuscripts in high impact journals and presented her works at international conferences.


Abstract

This study was aimed to determine the effect of sonication condition (time and temperature) on the milk/soy milk yogurt quality. Yogurt quality such as particle size, viscosity, texture, and syneresis of the milk/soy milk were analyzed in this study. The effects of both sonication time and temperature on the yogurts were analyzed using two ways ANOVA and post hoc Tukey’s test. The study showed that both sonication time and temperature did not significantly affect the particle size of milk/soy milk yogurt (p>0.05). Although there was a reduction in particle size of sonicated samples, it was insignificant. On the other hand, the viscosity of milk/soy milk quality was significantly affected by sonication time and temperature (p>0.05). Sonication time and temperature also significantly affect the milk/soy milk texture in terms of firmness, consistency, cohesiveness, and an index of viscosity. Sonicated samples have significantly higher textural quality as compared to control (p<0.05). In addition, syneresis of milk/soy milk yogurt was also significantly affected by the sonication time and temperature. Sonication condition (time and temperature) significantly affects viscosity, texture, and syneresis of milk/ soy milk yogurt but did not significantly affect its particle size.

Biography

Marco Iammarino is a Food Technologist and Chemical Surveyor. Since 2002, he is a Researcher at Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata (IZS-PB) of Foggia (Italy). Currently, he is employed as Principal Investigator at National Reference Centre for Detection of Radioactivity in Feed and Foodstuff of IZS-PB. He deals about food quality and safety, analytical chemistry applied to food analysis, research & development and analytical methods validation. In particular, he has developed several analytical methods (HPLC, HPIC, CE, LSC, TLC, ELISA) for the determinations of food additives (nitrites, nitrates, sulphites, polyphosphates, organic acids, etc.), radionuclides, mycotoxins, pesticides and drug residues in foods and feed materials. He has published more than 100 articles in peer-reviewed and academic journals, congress proceedings and books. The H index has reached 10 and citation exceeded 235 (source: Scopus). He was a reviewer for 49 international journals.


Abstract

Control of the presence of food additives in meat products is important to ensure their quality and safety. However, the attention of the official control plans is usually more focused on identifying/quantifying some food preservatives (i.e. nitrites/nitrate and sulphites) with respect to food dyes. The use of food dyes in meat is regulated by the actual normative due to several food safety concerns. Only three food dyes (cochineal, carminic acid, carmines (E120), Ponceau 4R, cochineal Red A (E124) and Allura Red AC (E129)) are admitted in some types of meat products, and maximum admissible levels were established in the Regulation No. 1129/2011/EC. Furthermore, the presence of different red dyes, including some carcinogens, was found in some types of spices used in the production of meat products (European Commission decision 2004/92/EC). For these reasons, an accurate method combining high performance liquid chromatography with UV-diode array detection was developed in this study, for the simultaneous determination of 12 food dyes (Amaranth, Ponceau 4R, Carmine, Ponceau SX, Ponceau 3R, Allura Red AC, Carmoisine, Erythrosine, Sudan I, Sudan II, Sudan III and Sudan IV) in meat products. The chromatographic separation was accomplished by a Hypersil Gold column (Thermo Fisher Scientific, 150 x 4.6 mm, i.d. 5 μm), eluted with an optimized step-change gradient, based on a mobile phase consisting of a acetate buffer and acetonitrile. These conditions guaranteed a very good selectivity towards endogenous interfering substances. The extraction of analytes from the matrix was accomplished using acetonitrile, methanol, water, ammonia, 50:40:9:1 (v/v/v/v) as solvent, and ultrasonic bath. Good analytical performances were obtained, in terms of selectivity, sensitivity, accuracy and ruggedness. Both method precision (CV% range: 6.2% - 18.0%) and recovery percentages (range: 86.4% - 105.0%) resulted in compliance with Decision 657/2002/EC, demonstrating that the procedure can be applied successfully for confirmation analyses.

Biography

Azmil Haizam Ahmad Tarmizi is a Senior Research Officer at the Malaysian Palm Oil Board. He joined the organisation in 2004 after obtaining his Master’s Degree in Bioprocess Engineering from the Universiti Teknologi Malaysia. He earned his Doctorate’s Degree in Food and Nutritional Sciences from the University of Reading UK in 2012 and awarded with a Master’s degree in Business Administration from Anglia Ruskin University UK in 2017. His major research areas are on the frying process and technology, quality and safety of oils and fats, palm oil processing, and palm based reference materials. He has published more than 20 peer reviewed and technical papers, and presented many papers in local and international conferences. He is also a member of the International Standardisation – Malaysia Mirror Committee on ISO/TC34/SC11: Animal and Vegetable Fats and Oils, and the Industry Standard Committee on Food Products and Food Safety (ISC U).


Abstract

Measurement of total polar compounds (TPC) is recognised as one of the indicator used to determine the thermal stability of oils when exposed to heat treatments. Nevertheless, dependency on TPC alone in judging oil quality and thus on safety is often deceptive, primarily for oils that contain higher level of natural diacylglycerols (DAG). Hence, this study investigates the levels of polar compound fractions in various oils when subjected to extended frying conditions. Four individual oils, i.e. palm olein (POO), soybean oil (SBO), canola oil (CAN) and sunflower oil (SFO), three binary blends of POO with SBO, CAN and SFO, respectively, and one tertiary blend containing POO, SBO and CAN were subjected to 144 frying cycles over 72 h of heating (8 h day-1 for 9 days). Polar compounds were initially separated gravimetrically through two elution steps before being introduced to the High Performance Liquid Chromatography (HPLC) – Size Exclusion Chromatography with Evaporative Light Scattering Detector (ELSD). It is much anticipated that the initial content of TPC in POO is considerably higher because of diacylglcerols (DAG) content of almost 7% when compared to other liquid oils that contained lesser amount of DAG (2%). After 9 days of frying, the TPC content in SFO reached 32% while other oils fell within 24 and 29%. Regardless to oil compositions, the levels of oxidised triacylglycerols (OxTAG) were ranged between 8 and 10%. The formation of polymerised triacylglycerols (PTAG) was more prominent when POO exhibited much lower PTAG (8%) as opposed to CAN (13%), SBO (17%) and SFO (19%). This study indicates that oils with higher unsaturation are prone to thermal degradation as a result of tertiary oxidation and/or thermal alteration of TAG molecules. Of course, blending liquid oils with POO provides the opportunity to moderate the level of PTAG when oils are heated at frying temperatures.

Biography

Balsano Clara was the Director of the Institute of Molecular Biology and Pathology (IBPM) of Cancer National Research (CNR) till last year June. She has a great research expertise on liver diseases, as highlighted by her scientific publications: author of more than 100 scientific papers. IF > 562; H-index: 33 (WOS); G-index: 60 (WOS); Citations > 3500 (WOS); orcid. org/0000-0002-9615-7031. In the last period, her research interest was dedicated in understanding of the molecular bases of the pathogenesis of Non-Alcoholic Fatty Liver Disease (NAFLD) to find new targets to develop new opportunities for a target therapy. NAFLD affects about 1.8 billion of people, the 25% of world population. Its prevalence is growing rapidly, along with the associated risk of worsening, in fact, the related cardiovascular risk cannot be underestimated. Currently, NAFLD should be considered one of the most important problems for National Health burden.


Abstract

Introduction: Diet significantly affects health. Incorrect feeding results in overweight or obesity that are associated with liver steatosis, a pathological condition that affects about 30% of the world's population. This pathology, characterized by accumulation of fat in the liver, inflammation and balloning, can progress toward cirrhosis and hepatocarcinoma (HCC). In such processes, transition metals (copper, zinc, Fe etc,) play a key role. Accordingly, altered copper homeostasis has been observed in various stages of Nonalcoholic fatty liver disease (NAFLD). Mediterranean diet has beneficial effects on health, and olive oil is one of the main actors. Oleuropein (Ole), a phenolic compound derived from green olives and olive leaves, is able to bind copper. Hence, we evaluated the correlation between Ole and intracellular copper concentration and its role in counteracting liver damage related to high fat diet intake (HFD). Methodology: Real-time PCR, atomic absorption, BioPlex multiplex biometric ELISA-based immunoassays, adipoRed and Western blots were performed for in vitro and in vivo experiments. Results: In vitro, fatty acids induce intracellular copper modulation. Oleuropein leads to a significant reduction in both the intracellular content of Cu and lipid accumulation. In a HFD mouse model treated with Ole, we highlighted a significant reduction in levels of Cu in liver tissue, hepatic steatosis and related inflammatory conditions. In particular, the levels of chemokines MCP1 and CXCL1, both correlated with the progression of liver disease, were significantly reduced. The involvement of genes (e.g. tp53, Myc etc.) in the control of the entry and efflux of copper in cells, is under investigation. Discussion: Our results demonstrate that Ole has inhibitory effects on the progression of liver disease that correlates with its ability to modulate copper.

Day2: May 15, 2018

Keynote Forum

Biography

Rob te Biesebeke has his PhD from the Wageningen University (NL), a MSc from the Utrecht University (NL). He is a Laboratory Engineer in Saxion Polytechnics (NL) with a track record of achievements in Microbial Biotechnology, Food technology, Nutrition, Dietary Fibers, Digestive Enzymes, Probiotics, Antimicrobials and Microbiology. He is a Technology and Science Officer at HNM Engineering. He was a former Microbial Biochemistry Researcher of the Ecole Polytechnique l’X (Paris, France), former Probiotics Taskforce for ILSI Life Sciences (Brussels, Belgium) and External Expert for the World Health Organization (Geneva, Switzerland).


Abstract

The human and animal microbiome is an incredible diverse and agile complex of microbial species with an important impact on the host. In humans and animals this relation is relevant for health and wellness. The microbiome of any living organism catalyzes biochemical reactions influencing the bioavailability and metabolism of bioactive molecules like nutraceuticals, pharmaceuticals, and feed and/or food additives. Consumption of food and these (bioactive) components changes the microbiome in that the growth of certain microbial species will be (dis) favored. Since the discovery of the different antibiotics that all have wide range antimicrobial impact, the research focus has been on the avoidance of communicable diseases. Almost all important bacterial infections are becoming resistant to antibiotics and to revert this phenomenon has become a major challenge. The smart use of antibiotics and new strategies to control microbial growth are key to controlling the spread of resistance. Every living organism has to deal with the reality that we live in a co-evolved symbiotic relationship with environmental microbes. There are now many examples showing a correlation between the microbiome, health and disease. The gastro-intestinal microbial species of the microbiome have been correlated with conditions like Inflammatory Bowel Disease, metabolic disorders, colorectal cancer, allergy and autism. The microbiological, nutritional and nutraceutical interventions facilitate a promising and underexplored opportunity to ‘engineer’ the Microbiome to prevent or treat a medical conditions.

Biography

Marco Pellegrini received his M.Sc. in Telecommunications Engineering from University of Firenze (Italy) at the age of 26 and completed his Ph.D. in Methods and Technologies for Environmental Monitoring at the age of 31 (University of Basilicata, Italy). He is a contract Professor of Physics and Remote Sensing, both at Universita Politecnica delle Marche (Italy) and is also a professional Telecommunications and Systems Engineer. He has published more than 20 papers in reputed journals and conference proceedings and has been serving as a peer reviewer and technical program committee (TPC) member for international conferences.


Abstract

According to the 2017 revision of the World Population Prospects database by the United Nations, the current world population of 7.6 billion is expected to grow reaching 9.8 billion in 2050 and 11.2 billion in 2100, so the food supply also will have to increase. Food production and security are major issues for feeding an increasing world population. New technology is being developed to allow for improving food production, safety and quality. Food engineering encompasses a wide range of activities in a multidisciplinary approach. It combines microbiology, applied physical sciences, chemistry and engineering for food and related industries. In the development of food engineering, one of the many challenges is to employ modern tools, technology and knowledge to develop new products and processes. In the framework of food engineering, it is widely recognized that information and communications technology (ICT) today plays a key role in ensuring a sustainable and inclusive growth of agriculture. ICT is a wide term that includes micro and nano-electronics, signal processing, communications, remote sensing, control systems, big data and biosystems; nano-materials, low-cost miniature and wearable sensors, satellite and airborne imaging, robotics and big data analytics are some of the applications that ICT can offer to the agricultural and food sector. In this work we first review the state-of-the-art of using ICT in food engineering and then draw some perspectives on possible evolutions of food processing that could improve the level of safety and automation for the food industry. In particular, we focus on the integration between 5G (the next generation of wireless communication networks that will have much higher transmission speeds and capacity and much lower latency than existing cellular systems), Internet of Things (IoT), artificial intelligence (AI) and machine learning (essential to make 5G a smarter network), big data collection and processing systems.

Tracks

  • Agriculture | Food Microbiology | Food Preservation | Nutrition | Food Engineering | Food Safety and Hygiene | Food Processing | Food Packaging
Location: Olympica 1

Sandra Poirier

Middle Tennessee State University, USA

Chair

Marco Pellegrini

Universita Politecnica delle Marche, Italy

Co Chair

Biography

Muhammad Wasim Sajid has completed his PhD from University of Agriculture Faisalabad Pakistan and 6 months Pre-doctoral studies from Newcastle University, UK. He is working as an Assistant Professor in COMSATS IIT Pakistan. He has published more than 11 papers in reputed journals, two book chapters and recently writing book on Food Safety. He has been serving as Head of new scientific organization, “International Association of Researcher in Applied Sciences (IARASC)”.


Abstract

In Pakistan, in the national agenda of economic development and milk production, the livestock contributes a significant share and occupies a distinct position. Pakistan is blessed with high yielding milch animals. Pakistan is enjoying fourth position among milk producing countries of the world behind India, China and USA. Regarding buffalo milk production Pakistan ranks second in the world. At small scale level, small farmers mainly contribute towards total milk production in the country. They do not have adequate conditions for the storage of feed/forage on their dairy farms due to economic backwardness. They are using different kind of pesticides on fodder as a protection against pests, ultimately these pesticides residues becomes part of animal body than milk. Pesticides residues presence in milk is alarming situation for all governing authorities and milk producing companies. Lactating cows and buffalos like other animals may be exposed to pesticides from feed with contamination; pesticide residues can be accumulated in various parts of body including milk. However, pesticide application to the animal’s body or their use in the animal shed, or even in the milk processing locations can also be a path of their exposure to pesticides and contamination to milk. To calculate the pesticide residues in buffalo milk a study was planned. Milk samples were collected from local dairy farms extracted for pesticide residues using glass column and was screened by HPLC. Milk samples were found contaminated with acephate, chlorpyriphos, fenthion, monocrotophos, profenofos and dimethoate. Methamidophos residues were not found in any of milk samples. The contamination rate of profenofos residues was 100% in two zones of milk collection. All milk samples were found contaminated but were below their MRL (Maximum Residual Limit). This study provides database to the government to establish their own MRL.

Biography

Elissa Ephrem has received her Master’s degree in Bio-health: Bioactive Molecules from the Lebanese University in 2013, with a thesis focusing on the effective encapsulation of rosemary essential oil, which was conducted at Laboratoire d’Automatique et de Génie des Procédés at the University of Claude Bernard Lyon I, France. She is currently a third-year PhD student working on the encapsulation of antibacterial agents for fruit juice preservation. Her PhD project is being conducted in collaboration between the Bioactive Molecules Research Laboratory at the Lebanese University and Laboratoire d’Automatique et de Génie des Procédés at the University of
Claude Bernard Lyon I, France.


Abstract

Fresh fruit juices are commonly susceptible to spoilage by acid-tolerant microorganisms among which lactobacilli, such as Lactobacillus fermentum. In this study, L. fermentum survival and growth in culture medium (MRS) and in fresh orange juice were challenged by the addition of a naturally occurring sesquiterpene alcohol, nerolidol. This compound is used as a food-flavouring agent. Its potent antibacterial activity had been demonstrated against several microorganisms. However, nerolidol is poorly soluble in water and photolabile, and is therefore unstable in aqueous media. To overcome this limitation, different formulations of nerolidol, in cyclodextrin (CD), drug-in-cyclodextrin-in liposome (DCL) and conventional liposome were considered. Free nerolidol,
added with DMSO as a co-solvent at 0.5% in final culture, exhibited a strong antibacterial activity within 22 hours in MRS at 37°C , with low minimal inhibitory (25 μM) and bactericidal (50 μM) concentrations. However, under refrigerated conditions (4°C), nerolidol showed no bactericidal activity within 22 hours in MRS, even at 200 μM. In orange juice, stored at 4°C, nerolidol had only shown a total bactericidal activity when added at 2000 μM, after 96 hours of incubation. At this concentration, CD/Ner inclusion complex showed a total bactericidal activity after 168 hours. The delay in activity could be due to the slow release of nerolidol incorporated into the inclusion complex. Our preliminary results have also shown that nerolidol encapsulated in DCL and conventional liposomes exhibited no antibacterial activity for up to 20 days of incubation. This could be due to the strong retention of nerolidol within DCL and conventional liposome systems. Current studies are being conducted to optimize DCLs and liposome formulations to enhance the release of nerolidol in fruit juice.

Biography

Filippo Michelino is a temporary Research Assistant at the University of Padua, where he has worked since 2016. His research is focused on the study of alternative processes for food drying and pasteurization under the supervision of Prof. Sara Spilimbergo. He attended the 2016 European Summer School in High Pressure Technologies. In 2016 he earned the Master degree in Chemical and Process Engineering, with a dissertation on”In situ Raman monitoring of dense CO2 drying of biological matrices”. He is first author of two papers in international peer reviewed journals.


Abstract

Food drying is one of the oldest processes for food preservation. Lowering the water content from foodstuff reduces enzymatic activity and microbial proliferation, thus improving food shelf life. Thermal treatment is the most widely used process for food drying; however, high temperatures induce losses of the matrix’s original properties, mainly physical/chemical and sensorial. Therefore, the development of alternative drying techniques has always been one of the main objectives of the industry. The present work investigates a newly-developed technology for food drying in mild conditions using carbon dioxide at supercritical state (SC-CO2). SC-CO2 extracts water from food matrixes at low temperatures, guaranteeing the preservation of both nutritional content and sensorial properties. SC-CO2 can be recycled within the process with a drastic reduction of CO2 consumption, hence cutting costs. Process optimization is dependent on the food matrix, as well as process parameters: temperature, pressure, CO2 flowrate, and treatment time influence the final sample’s weight loss and water activity. Overall, this study demonstrates that SC-CO2 is a promising alternative to traditional food drying methods.

Biography

Giovanni Lorenzon is a Chemical Engineer graduated in 2017 with a master thesis on environmental-friendly strategies for the exploitation of beer’s industrial residues through anaerobic digestion and nanocellulose synthesis. Since then, he worked as a Research Fellow in the Department of Chemical Engineering of the University of Padua, under the supervision of Prof. Sara Spilimbergo and Prof. Andrea Santomaso. His main research focus regards the optimization of the supercritical-CO2 drying process, its exploitation for powder production and the subsequent particle analysis by means of computer-based simulations.


Abstract

Introduction: The present work investigates the feasibility of an innovative mild drying process using CO2 at supercritical state (SC-CO2).The efficiency of the process was proven in terms of microbiological and enzymatic stability, micronutrients preservation, water activity and colour in the treated products. Moreover, the possibility of further processing the dried samples into powder was investigated, in order to produce innovative food ingredients and additives.
Materials & Methods: Experiments were carried out on different food products using a high-pressure cell at 10-14 MPa, different temperatures, never higher than 50°C, and drying times to establish the influence of each parameter on the final results. SC-CO2-dried samples were evaluated in terms of (i) mesophilic bacteria, pathogens, yeast and molds inactivation through standard plate count, (ii) enzymatic content through spectroscopic techniques, (iii) carotenoid, anthocyanin, vitamin and flavonoid concentration through HPLC and GC-mass instrumentation, (iv) water activity and (v) total color difference through reflectance probe.
Results & Conclusion: The results demonstrated that SC-CO2 drying was a promising green drying technology that combines drying and pasteurization in just one single step with a significant impact on both safety and costs. 

Biography

Sandra Grebenteuch has studied Food Chemistry at the Technical University of Munich. Presently, she is doing a PhD at the Berlin Institute of Technology. As part of the NutriAct cluster, she investigates the oxidative stability of polyunsaturated fatty acids in model and food systems. “NutriAct” means: Nutritional Intervention for Healthy Aging: Food Patterns, Behavior, and Products. Its ambitious aim is to transform research results on consumer’s food choices and its consequences into a substantial improvement of dietary intake of the adult population through both, a behavioral strategy and new products.


Abstract

In this day and age, nutrition plays an essential role in our lives. There is an increasing desire for foods which have a health-promoting and disease preventing effect. The attention of consumers is brought to the ratio of unsaturated ω-3 and ω-6 fatty acids (PUFAs) within food. Rapeseed oil has a higher content of linolenic acid in comparison to other vegetable oils. In conjunction with the research project (NutriAct), we are investigating the oxidative stability of rapeseed oil in various food matrices and under different process conditions. Unsaturated fatty acids rapidly degrade to volatile compounds. Hence, the stability of oil in food systems must be investigated, especially if food is enriched with ω-3 and ω-6 fatty acids, so that the health promoting effects remain. The degradation products formed by lipid oxidation are of interest because they decisively influence the quality of food. By using various analytical methods, the oxidative deterioration can be observed. For example the volatile compounds are analysed by using GC-MS and the technique of Headspace (HS). The rancidity of rapeseed oil is characterized by certain marker substances: Hexanal, 2-Hexenal, 2, 4-Decadienal, among others. The research focus is on the various influences on lipid oxidation. The external parameters consider temperature, storage time or water content, whilst the internal parameters consider the composition of oil and interactions with other food compounds. The rate of oxidative deterioration is predominantly affected by temperature and the content of antioxidants, such as tocopherols and sinapinic acid. The aim of the study of the food matrix is to ensure the highest possible oxidative stability of the essential ω-3 and ω-6 fatty acids during the production, storage and treatment of foods as well as to characterise the chemical interactions of degradation products of lipid oxidation with other food ingredients.

Biography

L Aumeistere has a professional Bachelor's Degree in Health Care (Dietitian) and also earned a Master of Engineering Degree in Food Science (Mg.Sc.ing.). Currently she is a PhD student in Food Science programme at Latvia University of Agriculture and also a Researcher in the Research Institute of Food Safety, Animal Health and Environment “BIOR”.


Abstract

Zinc is essential component and plays crucial role in metabolism processes; therefore insufficient zinc intake at young age can detrimentally affect growth and development. More than half of the infants in Latvia (~57%) are exclusively breastfed for the first six months of life. The aim of this research was to determine zinc content in mature breast milk among lactating women in Latvia. The study was conducted from November 2016 until December 2017. In total, 64 mature milk (at least 1 month postpartum) samples pooled within 24 h were collected. Participants (64 mothers with singleton deliveries of 34 boys and 31 girl) were 23 to 38 years old. Babies were 1.5 to 21 month old. More than half of the participants (n=39) were exclusively breastfeeding during the study, while 25 mothers had started the weaning. Zinc content was determined using inductively coupled plasma mass spectrometry (ICP MS Agilent 7700x, Japan). In total, zinc content in breast milk varied from 0.01 to 0.34 mg 100 ml-1 with a mean content 0.12±0.08 mg 100 ml-1. Arranging results according to child’s age, we observed that zinc content in breast milk significantly decreases after six months (see Table 1). Comparing our data to prescribed AI (adequate intake) and RDA (recommended daily allowance) for zinc (see Table 1), we conclude that infants in Latvia might not receive sufficient zinc intake with breast milk. To draw further conclusion, mother’s dietary habits during lactation period should be analysed as they may reveal some influence on zinc content in breast milk. In addition, zinc serum levels should be assessed, both mothers and children.

Biography

Elham Nasiri has completed her MSc from Food Technology Department of Shiraz University and is currently pursuing her PhD in Technology of Food Science in Tabriz University. She was a Teaching Assistant in Sarvestan Azad University for 2 years and has been a Research Assistant in FAU University of Erlangen (Germany) since 2017. She has published 3 papers in reputed journals and presented one speech and 20 papers in valid congress.


Abstract

Shrimps are nutritious foods that are highly susceptible to microbial and physicochemical deteriorations, such as Shrimp melanosis (black spot). Sulphiting agents are widely used to prevent melanosis and microbial deterioration in shrimp; but the hazards related to sulphated foods, have created a necessity to find the effective natural alternatives. Lemon peel is the waste product of juice industry which is usually disposed in landfills or used as animal feed in small quantities. In the other investigation we observed the antityrosinase effect of lemon peel essential oil (LPEO) on shrimp. Essential oil application as food preservatives is often limited due to flavouring considerations. So by incorporation in coatings smaller amounts of EOs would be needed due to a gradual release on food surfaces; moreover nanoemulsions of EOs have more stability and efficiency. The objective of this study was to assess the combined effect of Lemon peel Nano EO (LPNEO) and carboxymethyl-cellulose (CMC) coating on improving postharvest quality of pacific white shrimp (Litopenaeus vannamei) during 10 days of storage at 4°C. Shrimps were immersed in CMC (1%) and CMC (1%) containing 0.5% LPNEO (Shrimp:CMC ratio 1:1 w/v), while sodium metabisulfite (SMS) and water were used as positive and negative controls respectively. The lower rate of the increase in Enterobacteriaceae, mesophilic, psychrophilic and lactic acid bacterial counts were observed in shrimps treated with LPNEO. The increase in thiobarbituric acid level, total volatile basic nitrogen and trimethylamine content were retracted as well in these samples compared to the other groups. The lowest melanosis formation was observed in LPNEO treated shrimps followed by CMC and SMS groups. Shrimps treated with LPNEO had also the significant higher sensory score for odour, colour and texture. The results suggest that the LPNEO incorporated in CMC may be promising as a new ecofriendly preservative to extend the shelf life of shrimps during refrigerator storage.

Biography

Ramírez-Sucre M O works on rheological/physicochemical evaluations. He was a member of the National Research System (Mexico) from 2013 to 2016. He is a Coordinator of the Strategic Alliance for the Sustainable Development of the South Pacific Region (ADESUR) in Acapulco, Mexico in 2016 which deals with added value of tropical fruits. He has been four years in charge of the food pilot plant in the Southeast Unit of CIATEJ. He held responsibility of 5 projects, two with technological developments. He has 8 international publications related to rheological and mechanical properties, one patent application (MX/a/2014/015444: Microaspersion Drying Process of the Hesperidin/Cyclodextrin complex) and one academic exchange (Institute of Agrochemistry and Food Technology, Valencia, Spain) for the study of protein-hydrocolloid interactions. He guided 4 theses and he is coordinator of various subjects (Food Physics, Food Chemistry, and Economic Engineering).


Abstract

Texture is one of the most critical attribute when referring to crops maturation. In Mexico the Habanero chili (Capsicum chinense) of the Yucatan Peninsula achieves the appellation of origin (2010) which makes it susceptible for exportation. The compounds (like capsaicinoids) vary depending on the variety of chili, climate, maturation stage and type of soil. The aim of this study was to measure the texture in terms of hardness of 1) habanero chili fruits cultivated in three types of soils (lu'um): brown (Ch'ich), black (Box) and red (K'áankab) during the development of the plant/fruit, and of 2) orange chilies in a post-harvest study, for later correlation to the maturity state.
Materials & Methods: The hardness was measured using a downward compression polarity and a displacement and speed limit of 2.0 cm and 1.0 cm/min respectively, to chili fruits from greenhouse plants.
Results: Hardness was greater in fresh-cut orange chilies than in coloring (semi-mature) and green chilies, reaching average values of 50.0, 30.7 and 33.7 respectively, without significant differences between soils. However, a major production was observed in red soils (14 orange chilies vs. 8 of black and brown soils) (Fig. 1). The major hardness during storage was presented in 2nd week for black and red soils, rapidly than in brown soil (Fig. 2) Hardness of post-harvest study in commercial chilies showed a maximum of 52.2N in orange chilies, most of them remained green (Fig. 3). The hardness incremented even in old chilies (up to 98N) with an elastic and hard-to-penetrate skin.
Conclusion: Habanero plants develop texture with time in fresh-cut fruits. Two weeks were enough for the fruit hardness development (>50N). Hardness is recommended to measure chili texture until it reaches 50-60N; hardness more than 70N should be measured with a more sophisticated technique, registering the elasticity of the chili skin like texture profile analysis (TPA).

Biography

Roberto Saverio Souza Costa has completed his graduation from University State of São Paulo in Brazil (Unesp – campus Ilha Solteira) and Master’s studies form University State of São Paulo (Unesp – campus Jaboticabal). He is an Agronomist Engineer in Coordenadoria de Assistência Técnica Integral (CATI) and he works with family farmers in projects of São Paulo State Government. He has experience as an extensionist in the area of Plant Science, with emphasis on Management and Cultural Treatments, Plant Nutrition and Fertilization and also in the area of Topography and Rural Engineering. He is also a University Professor at Centro Universitário Unifafibe, in Bebedouro city, in the Agronomic Engineering course. He has published more than 8 papers in scientific journals and has 29 presentations of the work.


Abstract

CATI Leite is a project developed in the State of São Paulo by the Coordenadoria de Assistência Técnica Integral (CATI), which aims to transfer technology, develop dairy activity to pasture and make viable production in family farms. Thus, the objective of this work was to evaluate the rural farms in the Jaboticabal region (cities: Jaboticabal, Monte Alto and Vista Alegre do Alto, SP, Brazil) that adopted the intensive grazing management system and implemented the Cati Leite Project, between the years of 2012 and 2017. Initially the extensive pasture areas were mapped with GPS and divided into pickets with a period of occupation of 1 day, respecting the cycle of the existing pasture (mombaça and tifton). The areas with mombaça pasture were divided in 29 pickets and the tifton pasture in 19 pickets. After 5 years implantation of the project CATI Leite, the results demonstrated that the property of Jaboticabal increased from 82 to 495 L/day and Monte Alto property from 22 to 80 L/day and Vista Alegre Alto has increased from 350 to 1600 L/day. In this way, it is concluded that the implementation of the CATI Leite project provided the increase of significant production, proving to be a viable and profitable alternative for the family producer.